Recombinant Mouse Dmrt1 Protein, Myc/DDK-tagged

• Cat.No.: Dmrt1-2587M
• Product Overview: Purified recombinant protein of mouse full-length doublesex and mab-3 related transcription factor 1 (Dmrt1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Transcription factor that plays a key role in male sex determination and differentiation by controlling testis development and male germ cell proliferation. Plays a central role in spermatogonia by inhibiting meiosis in undifferentiated spermatogonia and promoting mitosis, leading to spermatogonial development and allowing abundant and continuous production of sperm. Acts both as a transcription repressor and activator: prevents meiosis by restricting retinoic acid (RA)-dependent transcription and repressing STRA8 expression and promotes spermatogonial development by activating spermatogonial differentiation genes, such as SOHLH1. Also plays a key role in postnatal sex maintenance by maintaining testis determination and preventing feminization: represses transcription of female promoting genes such as FOXL2 and activates male-specific genes. May act as a tumor suppressor. May also play a minor role in oogenesis.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 39.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dmrt1 doublesex and mab-3 related transcription factor 1 [ Mus musculus (house mouse) ]
• Official Symbol: Dmrt1
• Synonyms: DMRT1; doublesex and mab-3 related transcription factor 1; doublesex- and mab-3-related transcription factor 1
• Gene ID: 50796
• mRNA Refseq: NM_015826
• Protein Refseq: NP_056641
• UniProt ID: Q9QZ59