Recombinant Mouse Ddrgk1 Protein, Myc/DDK-tagged

• Cat.No.: Ddrgk1-2497M
• Product Overview: Purified recombinant protein of mouse full-length DDRGK domain containing 1 (Ddrgk1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Substrate adapter for ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, which plays a key role in reticulophagy (also called ER-phagy). In response to endoplasmic reticulum stress, promotes recruitment of the E3 UFM1-protein ligase UFL1 to the endoplasmic reticulum membrane: in turn, UFL1 mediates ufmylation of proteins such as RPN1 and RPL26/uL24, promoting reticulophagy of endoplasmic reticulum sheets. Ufmylation-dependent reticulophagy inhibits the unfolded protein response (UPR) by regulating ERN1/IRE1-alpha stability. Ufmylation in response to endoplasmic reticulum stress is essential for processes such as hematopoiesis or inflammatory response. Required for TRIP4 ufmylation, thereby regulating nuclear receptors-mediated transcription. May play a role in NF-kappa-B-mediated transcription through regulation of the phosphorylation and the degradation of NFKBIA, the inhibitor of NF-kappa-B. Plays a role in cartilage development through SOX9, inhibiting the ubiquitin-mediated proteasomal degradation of this transcriptional regulator.
• Molecular Mass: 36 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ddrgk1 DDRGK domain containing 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ddrgk1
• Synonyms: DDRGK1; DDRGK domain containing 1; DDRGK domain-containing protein 1; UFM1-binding protein 1 containing a PCI domain; Ufbp1; AI326138; RP23-100C5.9; 1110001I20Rik; 2600009E05Rik
• Gene ID: 77006
• mRNA Refseq: NM_029832
• Protein Refseq: NP_084108
• UniProt ID: Q80WW9