Recombinant Mouse Cyp2e1 Protein, Myc/DDK-tagged

• Cat.No.: Cyp2e1-2417M
• Product Overview: Purified recombinant protein of mouse full-length cytochrome P450, family 2, subfamily e, polypeptide 1 (Cyp2e1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.
• Molecular Mass: 57.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cyp2e1 cytochrome P450, family 2, subfamily e, polypeptide 1 [ Mus musculus (house mouse) ]
• Official Symbol: Cyp2e1
• Synonyms: CYP2E1; cytochrome P450, family 2, subfamily e, polypeptide 1; cytochrome P450 2E1; CYPIIE1; cytochrome P450-J; cytochrome P450-ALC; 4-nitrophenol 2-hydroxylase; cytochrome P450, 2e1, ethanol inducible; Cyp2e
• Gene ID: 13106
• mRNA Refseq: NM_021282
• Protein Refseq: NP_067257
• UniProt ID: Q05421