Recombinant Mouse Cyb5b Protein, C-Myc/DDK tagged

• Cat.No.: CYB5B-4122M
• Product Overview: Purified recombinant protein of Mouse cytochrome b5 type B (Cyb5b), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Predicted to enable enzyme activator activity and heme binding activity. Predicted to contribute to nitrite reductase (NO-forming) activity. Predicted to be involved in nitric oxide biosynthetic process. Located in mitochondrial inner membrane. Is expressed in several structures, including adrenal gland; craniocervical region bone; gut; neural retina; and urinary system. Orthologous to human CYB5B (cytochrome b5 type B).
• Molecular Mass: 16.3 kDa
• AA Sequence: MATPEASGSGEKVEGSEPSVTYYRLEEVAKRNSAEETWMVIHGRVYDITRFLSEHPGGEEVLLEQAGADATESFEDVGHSPDAREMLKQYYIGDVHPSDLKPKGDDKDPSKNNSCQSSWAYWFVPIVGAILIGFLYRHFWADSKSSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: > 0.05 μg/μL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10 % glycerol.

Gene Information

• Gene Name: Cyb5b cytochrome b5 type B [ Mus musculus (house mouse) ]
• Official Symbol: Cyb5b
• Synonyms: Cyb5b; cytochrome b5 type B; Cyb5m; 1810044O22Rik; cytochrome b5 type B
• Gene ID: 66427
• mRNA Refseq: NM_025558
• Protein Refseq: NP_079834
• UniProt ID: Q9CQX2

Q&As

Is there an iron or heme content for this particular product?

The culture medium contains trace elements such as iron (Fe). In theory, these elements should be incorporated into the structure. However, this has not been experimentally verified on the final product.