Recombinant Mouse Ctnnb1 Protein, MYC/DDK-tagged

• Cat.No.: Ctnnb1-779M
• Product Overview: Purified recombinant protein of full-length mouse catenin (cadherin associated protein), beta 1, complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes not only an important cytoplasmic component of the classical cadherin adhesion complex that forms the adherens junction in epithelia and mediates cell-cell adhesion in many other tissues but also a key signaling molecule in the canonical Wnt signaling pathway that controls cell growth and differentiation during both normal development and tumorigenesis. The gene product contains a central armadillo-repeat containing domain through which it binds the cytoplasmic tail of classical cadherins; meanwhile, it also binds alpha-catenin, which further links the cadherin complex to the actin cytoskeleton either directly or indirectly. Beta-catenin is therefore necessary for the adhesive function of classical cadherins. Another key function of this protein is to mediate the canonical Wnt signaling pathway and regulate gene transcription. Without Wnt signal, cytoplasmic beta-catenin that is not associated with the cadherin complex is quickly phosphorylated at the N-terminal Ser/Thr residues by the so called degradation complex containing axin, adenomatous polyposis coli (APC), casein kinase I, and GSK3B, then ubiquitylated by beta-TrCP, and degraded by the proteasome. However, in the presence of Wnt signal, the degradation complex is disrupted and the stabilized cytoplasmic beta-catenin translocates into the nucleus, where it binds various transcription factors and, together with these factors, regulates the transcription of many downstream genes. Mutations of this gene have been linked with various types of tumors. Alternatively spliced variants have been found for this gene.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 130.7 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ctnnb1 catenin (cadherin associated protein), beta 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ctnnb1
• Synonyms: Ctnnb1; catenin (cadherin associated protein), beta 1; Bfc; Mesc; Catnb; catenin beta-1; beta-catenin
• Gene ID: 12387
• mRNA Refseq: NM_007614
• Protein Refseq: NP_031640
• UniProt ID: Q02248