Recombinant Mouse Ctla4, Fc-His

• Cat.No.: Ctla4-1717M
• Product Overview: Recombinant Mouse Ctla4 was expressed inNSO cells. The DNA sequence encoding amino acid residues 37 - 162 of the extracellular domain of mouse CTLA-4 was fused to the Fc region of human IgG1 (with IEGR added at the amino-terminus and 6x his-tag added at the carboxy-terminus).

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc&His
• Protein Length: 37-162 a.a.
• Description: CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) also known as CD152, is a protein which is expressed on the surface of Helper T cells and plays an important regulatory role in the immune system. CTLA4 is a member of the immunoglobulin superfamily, expressed on the surface of Helper T cells. CTLA4 transmits an inhibitory signal to T cells. CTLA4 is potentially therapeutic in auto-immune diseases, such as rheumatoid arthritis, HIV, autoimmune thyroid disease, multiple sclerosis and may also be useful during organ transplantation and cancer treatment.
• Molecular Weight: The predicted molecular weight of Recombinant Mouse CTLA-4 is Mr 41 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 55 kDa.
• State Of Matter: Lyophilized.
• Purity: >97% by SDS Page and analyzed by silver stain.
• Formulation: 0.2 μm filtered and lyophilized from PBS.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: Measured by its ability to inhibit IL-2 production in Jurkat cells stimulated with 1 μg/ml rhB7-1/Fc in the presence of PHA. The expected ED50 for this effect is 0.1 - 0.4 μg/ml.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: Ctla4 cytotoxic T-lymphocyte-associated protein 4 [ Mus musculus ]
• Synonyms: Ctla4; cytotoxic T-lymphocyte-associated protein 4; Cd152; Ly-56; Ctla-4; CD152 antigen; OTTMUSP00000002168
• Gene ID: 12477
• mRNA Refseq: NM_009843
• Protein Refseq: NP_033973
• UniProt ID: P09793
• Chromosome Location: 1 C; 1 30.1 cM
• Pathway: Autoimmune thyroid disease; Cell adhesion molecules (CAMs); T cell receptor signaling pathway