Recombinant Mouse CSF1R protein(Met1-Ser511), His-tagged

• Cat.No.: Csf1r-337M
• Product Overview: Recombinant Mouse CSF1R (NP_001032948.2) (Met 1-Ser 511) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ser511
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized M-CSF Protein, Mouse, Recombinant (ECD) at 2 μg/mL (100 μL/well) can bind CSF1R Protein, Mouse, Recombinant (His Tag), the EC50 is 180-640 ng/mL.
• Molecular Mass: The secreted recombinant mouse CSF1R consists of 503 amino acids and has a predicted molecular mass of 56.8 kDa. As a result of glycosylation, the apparent molecular mass of rm CSF1R is approximately 80-90 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Csf1r colony stimulating factor 1 receptor [ Mus musculus ]
• Official Symbol: CSF1R
• Synonyms: CSF1R; colony stimulating factor 1 receptor; macrophage colony-stimulating factor 1 receptor; c-fms; CSF-1-R; CSF-1 receptor; proto-oncogene fms; proto-oncogene c-Fms; Fms; CD115; Csfmr; Fim-2; CSF-1R; M-CSFR; M-CSF-R; AI323359
• Gene ID: 12978
• mRNA Refseq: NM_001037859
• Protein Refseq: NP_001032948