Recombinant Mouse Crbn Protein, His-tagged

• Cat.No.: Crbn-838M
• Product Overview: Recombinant Mouse Crbn Protein (Lys303-Glu433) with His tag was expressed in E. coli.

Specification

• Description: This gene encodes a protein with a Lon protease domain, a "regulators of G protein-signaling" (RGS)-like domain and a leucine zipper. It has been proposed to regulate the assembly and surface expression of large-conductance calcium-activated potassium channels in brain and to bind thalidomide. In humans mutation in this gene causes autosomal recessive nonsyndromic cognitive disability. In mouse deficiency of this gene serves as a model to study the molecular mechanisms governing learning and memory as they relate to intellectual disability. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 18.4 kDa; Accurate Molecular Mass: 20&18 kDa
• Protein length: Lys303-Glu433
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Crbn cereblon [ Mus musculus (house mouse) ]
• Official Symbol: Crbn
• Synonyms: CRBN; cereblon; protein cereblon; protein PiL; novel lethal; piL; AF229032; AW108261; 2610203G15Rik; 2900045O07Rik
• Gene ID: 58799
• mRNA Refseq: NM_021449
• Protein Refseq: NP_067424
• UniProt ID: Q8C7D2