Recombinant Mouse Cluster Of Differentiation 14, His-tagged

• Cat.No.: Cd14-1385M
• Product Overview: Recombinant Mouse CD14 is produced from mouse CD14 transfectedCHO-cellsin serum free medium. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR). The recombinant mouse CD-14 was purified by His-tag and controled by SDS page.The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 is the 50 kDa protein.

Specification

• Cat. No.: Cd14-1385M
• Description: CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.
• Source: CHO cells.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Applications: • ELISA • Inhibition Assays • Western Blotting.
• Solubility: It is recommended to reconstitute the lyophilized Mouse CD14 in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Stability: Lyophilized Mouse CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse CD14 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: Cd14 CD14 antigen [ Mus musculus ]
• Synonyms: Cd14; Cluster of differentiation 14; CD14 antigen; CD14 molecule; Myeloid cell-specific leucine-rich glycoprotein; monocyte differentiation antigen CD14
• Gene ID: 12475
• mRNA Refseq: NM_009841
• Protein Refseq: NP_033971
• UniProt ID: P10810
• Chromosome Location: 18 B2; 18 31.0 cM
• Pathway: Hematopoietic cell lineage; MAPK signaling pathway; Regulation of actin cytoskeleton; Toll-like receptor signaling pathway
• Function: lipopolysaccharide binding; lipoteichoic acid binding; protein binding