Recombinant Mouse Cenpt Protein, Myc/DDK-tagged

• Cat.No.: Cenpt-2109M
• Product Overview: Purified recombinant protein of mouse full-length centromere protein T (Cenpt), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 56.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cenpt centromere protein T [ Mus musculus (house mouse) ]
• Official Symbol: Cenpt
• Synonyms: CENPT; centromere protein T; CENP-T; G630055P03Rik
• Gene ID: 320394
• mRNA Refseq: NM_177150
• Protein Refseq: NP_796124
• UniProt ID: Q3TJM4