Recombinant Mouse Cd80 Protein, hFc-tagged, FITC conjugated

Cat.No. : Cd80-6898MF
Product Overview : FITC conjugated recombinant Mouse Cd80 (Val38-Lys245) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).
Unit
Price
Qty
  • Specification
  • Gene Information
  • Related Products
  • Download
Description : B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response.
B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
Source : HEK293
Species : Mouse
Tag : Fc
Form : Lyophilized
Molecular Mass : The protein has a calculated MW of 50.3 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Val38-Lys245
N-terminal Sequence Analysis : Val 38
Endotoxin : < 1.0 EU/ μg by the LAL method.
Purity : > 95 % as determined by SDS-PAGE
Characteristic : Disulfide-linked homodimer
Labeled with FITC via amines
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage Buffer : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name Cd80
Official Symbol Cd80
Synonyms CD80; CD80 antigen; T-lymphocyte activation antigen CD80; B7 protein; activation B7-1 antigen; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
Gene ID 12519
mRNA Refseq NM_009855
Protein Refseq NP_033985
UniProt ID Q00609

Not For Human Consumption!

Inquiry

  • Reviews
  • Q&As

Customer Reviews (0)

Write a review

Q&As (0)

Ask a question

Ask a Question for All Cd80 Products

Required fields are marked with *

My Review for All Cd80 Products

Required fields are marked with *

0

Inquiry Basket

cartIcon