Recombinant Mouse CD40 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Cd40-325MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse Cd40 (NP_035741.2) (Met1-Arg193) was expressed with the Fc region of rabbit IgG at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Arg193， 405
• Description: CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
• Form: Lyophilized
• Molecular Mass: 45.4 kDa
• N-terminal Sequence Analysis: Leu 20
• Endotoxin: < 1.0 EU/ μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: Samples are stable for up to twelve months from date of receipt at -70 centigrade. Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Cd40 CD40 antigen [ Mus musculus ]
• Official Symbol: Cd40
• Synonyms: CD40; CD40 antigen; tumor necrosis factor receptor superfamily member 5; CD40L receptor; B-cell surface antigen CD40; T-cell differentiation antigen; tumor necrosis factor receptor superfamily, member 5; IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
• Gene ID: 21939
• mRNA Refseq: NM_011611
• Protein Refseq: NP_035741