Recombinant Mouse Cd33 Antigen Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Cd33-6889MAF555
• Product Overview: A DNA sequence encoding the extracellular domain of mouse Cd33 (Met 1 - Glu 240) (Alexa Fluor 555 conjugated) was fused with the Fc region of human IgG1 at the C-terminus. It comprises 465 amino acids and has a calculated molecular mass of 51.6 kDa.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: 465
• Description: Mouse myeloid cell surface antigen Cd33 is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Cd33 contains 1 Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain.
• Form: Lyophilized
• Molecular Mass: The secreted recombinant mouse Cd33/Fc is a disulfide-linked homodimer. The reduced monomer comprises 465 amino acids and has a calculated molecular mass of 51.6 kDa. As a result of glycosylation, the apparent molecular mass of rm CD33/Fc monomer is approximately 55 - 60 kDa in SDS-PAGE under reducing conditions.
• N-terminal Sequence Analysis: Gln 17
• Endotoxin: < 0.06 EU/ μg as determined by LAL test.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Gene Information

• Gene Name: Cd33 CD33 antigen [ Mus musculus ]
• Official Symbol: Cd33
• Synonyms: CD33; CD33 antigen; myeloid cell surface antigen CD33; sialic acid-binding Ig-like lectin 3; gp67; Siglec-3
• Gene ID: 12489
• mRNA Refseq: NM_001111058
• Protein Refseq: NP_001104528