Recombinant Mouse Cd276 protein(Met1-Phe244), His-tagged, Biotinylated

• Cat.No.: Cd276-1140M
• Product Overview: Biotinylated Recombinant Mouse CD276 (NP_598744.1) (Met1-Phe244) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Protein length: Met1-Phe244
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse CD276 comprises 227 amino acids and has a predicted molecular mass of 24.9 kDa. The apparent molecular mass of the protein is approximately 40.9 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd276 CD276 antigen [ Mus musculus ]
• Official Symbol: Cd276
• Synonyms: CD276; CD276 antigen; B7-H3; B7 homolog 3; costimulatory molecule; B7h3; B7RP-2; AU016588; 6030411F23Rik
• Gene ID: 102657
• mRNA Refseq: NM_133983
• Protein Refseq: NP_598744