Recombinant Mouse Carbonic Anhydrase 2, His-tagged

• Cat.No.: Car2-1191M
• Product Overview: A DNA sequence encoding the mouse Car2 ( AAH55291.1 ) ( Met 1 ?C Lys 260 ) was expressed, with a C-terminal polyhistidine tag

Specification

• Species: Mouse
• Source: Mouse
• Tag: His
• Protein Length: 1-260 a.a.
• Description: Carbonic anhydrases ( Cas ) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature.
• Form: Lyophilized from sterile 50 mM Tris, 150mM NaCl , pH 7.5 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Purity: > 96 % as determined by SDS-PAGE
• Endotoxin: μ1.0 EU per μg protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70 μ
• Predicted N terminal: Ser 2
• Molecular Mass: The recombinant mouse Car2 comprises 270 amino acids and has a calculated molecular mass of 30.4 kDa. The recombinant protein migrates as an approximately 30-33 kDa band in SDS-PAGE under reducing conditions.
• Storage: Store it under sterile conditions at -70μ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Pathways: Bile secretion; Collecting duct acid secretion; Gastric acid secretion; Nitrogen metabolism; Pancreatic secretion

Gene Information

• Gene Name: Car2 carbonic anhydrase 2 [ Mus musculus ]
• Official Symbol: Car2
• Synonyms: Car2; Ca2; CAII; Car-2; Ltw-5; Lvtw-5; AI131712; 3 A1; 3 10.5 Cm; carbonic anhydrase 2; CA II; CA-II; carbonic anhydrase II; carbonate dehydratase II
• Gene ID: 12349
• mRNA Refseq: NM_009801
• Protein Refseq: NP_033931
• UniProt ID: P00920
• Chromosome Location: 3 A1; 3 10.5 cM
• Function: carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding