Recombinant Mouse Car9 protein(Met1-Asp390), His-tagged

• Cat.No.: Car9-914M
• Product Overview: Recombinant Mouse CAR9 (NP_647466.2) (Met 1-Asp 390) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Protein length: 1-390 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its esterase activity. The specific activity is >100 pmoles/min/μg.
• Molecular Mass: The secreted recombinant mouse CAR9 comprises 370 amino acids and has a calculated molecular mass of 40.3 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 45-50 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Car9 carbonic anhydrase 9 [ Mus musculus ]
• Official Symbol: Car9
• Synonyms: CAR9; carbonic anhydrase 9; CA-IX; carbonic anhydrase IX; carbonate dehydratase IX; membrane antigen MN homolog; Ca9; CAIX; MN/CA9
• Gene ID: 230099
• mRNA Refseq: NM_139305
• Protein Refseq: NP_647466