Recombinant Mouse BTLA protein(Met1-Gly176), hFc-tagged
Cat.No. : | BTLA-8698M |
Product Overview : | Recombinant Mouse BTLA (AAI08964.1, with mutations K173E) (Met1-Gly176) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus. |
Availability | March 29, 2025 |
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Species : | Mouse |
Source : | HEK293 |
Tag : | Fc |
Protein Length : | Met1-Gly176 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Immobilized mouse BTLA-Fc at 10 μg/ml (100 μl/well) can bind biotinylated mouse HVEM-Fch, The EC50 of biotinylated mouse HVEM-Fch is 15.7-36.7 ng/ml. |
Molecular Mass : | The recombinant mouse BTLA /Fc is a disulfide-linked homodimer. The reduced monomer comprises 388 amino acids and has a predicted molecular mass of 437 kDa. The apparent molecular mass of the protein is approximately 57-62 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 90 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name | Btla B and T lymphocyte associated [ Mus musculus ] |
Official Symbol | BTLA |
Synonyms | BTLA; B and T lymphocyte associated; B- and T-lymphocyte attenuator; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein; A630002H24; MGC124217; MGC124218; |
Gene ID | 208154 |
mRNA Refseq | NM_001037719 |
Protein Refseq | NP_001032808 |
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Not For Human Consumption!
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