Recombinant Mouse BCL2-associated X Protein

• Cat.No.: Bax-244M
• Product Overview: Recombinant Mouse Bax produced inE.Coliis a single, non-glycosylated polypeptide chain. The Mouse Bax contains 133 (38-171) amino acids. The Mouse Bax is purified by proprietary romatographic techniques.

Specification

• Cat. No.: Bax-244M
• Description: The Bcl-2-associated X protein, or Bax is a protein of theBcl-2gene family. It promotesapoptosisby competing withBcl-2proper. Bax is a pro-apoptotic Bcl-2 protein containing BH1, BH2 and BH3 domains. In healthy mammalian cells, the majority of BAX is found in thecytosol, but upon initiation of apoptoticsignaling, Bax undergoes a conformation shift, and inserts intoorganellemembranes, primarily the outermitochondrialmembrane. Bax is believed to interact with, and induce the opening of the mitochondrial voltage-dependent anion channel,VDAC. Alternatively, growing evidence suggest that activated Bax and/orBakform an oligomeric pore,MACin the outer membrane. This result in the release ofcytochrome cand other pro-apoptotic factors from the mitochondria often referred to as mitochondrial outer membrane permeabilization, leading to activation ofcaspases.
• Source: Escherichia Coli.
• Physical Appearance: Sterile Filtered colorless solution.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Formulation: The protein, 200µg/ml contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.
• Other Available Varients: Recombinant Mouse BID, Recombinant Mouse tBID, Recombinant Mouse GST-BID Recombinant Mouse GST-Bax, Recombinant Mouse GST-Bax BH1, Recombinant Mouse GST-Bax BH2, Recombinant Mouse GST-Bax BH3, Recombinant Mouse GST-Bax IGDE, Recombinant Mouse GST-Bax D/A in IGDE.
• Endotoxin: It is less than 0.1 ng/µg of rmBax.
• Applications: Input marker or positive control (Western Blotting). Function study (binding assay).
• Stability: The rMuBax although stable at 12°Cfor 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
• Tag: Non
• Protein length: 38-171

Gene Information

• Gene Name: Bax BCL2-associated X protein [ Mus musculus ]
• Synonyms: Bax; BCL2-associated X protein
• Gene ID: 12028
• mRNA Refseq: NM_007527
• Protein Refseq: NP_031553
• UniProt ID: Q07813
• Chromosome Location: 7 B5; 7 23.0 cM
• Pathway: Amyotrophic lateral sclerosis (ALS); Apoptosis; Colorectal cancer; Huntington"s disease; Huntington"s disease; Neurotrophin signaling pathway; Pathways in cancer; p53 signaling pathway; Prion diseases
• Function: BH3 domain binding; protein binding; protein heterodimerization activity

Reviews

01/07/2020

Reliable partner for protein expression and analysis.

01/23/2019

Swift and reliable protein purification services.

01/18/2018

Consistency in results, a hallmark of their service.

Q&As

Are genetic mutations in BAX linked to any diseases?

Some genetic mutations in BAX are associated with cancer.

What therapeutic strategies are being explored involving BAX?

Strategies include modulating BAX activity for cancer therapy and neurodegenerative diseases.

How does BAX interact with other members of the Bcl-2 protein family?

BAX can form heterodimers with anti-apoptotic Bcl-2 proteins, or homodimers to promote apoptosis.

What happens when BAX is dysregulated?

Dysregulation can lead to excessive cell death or contribute to cancer.

What is BAX's role in cellular apoptosis?

BAX promotes apoptosis by permeabilizing the mitochondrial membrane.

What triggers the activation of BAX?

Stress signals or cellular damage can trigger BAX activation.

How is BAX regulated within the cell?

It is regulated by various members of the Bcl-2 family and other signaling pathways.