Recombinant Mouse Babam1 Protein, Myc/DDK-tagged

• Cat.No.: Babam1-1814M
• Product Overview: Purified recombinant protein of mouse full-length BRISC and BRCA1 A complex member 1 (Babam1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. In these 2 complexes, it is probably required to maintain the stability of BABAM2 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36 component. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression. Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 36.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Babam1 BRISC and BRCA1 A complex member 1 [ Mus musculus (house mouse) ]
• Official Symbol: Babam1
• Synonyms: Babam1; BRISC and BRCA1 A complex member 1; Merit40; 5430437P03Rik; BRISC and BRCA1-A complex member 1; BRCA1-A complex subunit MERIT40; mediator of RAP80 interactions and targeting subunit of 40 kDa; new component of the BRCA1-A complex
• Gene ID: 68251
• mRNA Refseq: NM_026636
• Protein Refseq: NP_080912
• UniProt ID: Q3UI43