Recombinant Mouse Apc Protein, His&SUMO-tagged

• Cat.No.: Apc-201M
• Product Overview: Recombinant Mouse Apc Protein (Ala2529-Ser2841) with N-His&SUMO tag was expressed in E. coli.

Specification

• Description: Enables several functions, including beta-catenin binding activity; dynein complex binding activity; and enzyme binding activity. Involved in insulin receptor signaling pathway; positive regulation of cold-induced thermogenesis; and positive regulation of protein localization to centrosome. Acts upstream of or within several processes, including animal organ development; regionalization; and regulation of microtubule polymerization or depolymerization. Located in several cellular components, including axonal growth cone; cell projection membrane; and microtubule cytoskeleton. Part of Scrib-APC-beta-catenin complex. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; peripheral nervous system ganglion; and retina. Used to study breast cancer; familial adenomatous polyposis; gastrointestinal system cancer (multiple); and reproductive organ cancer (multiple). Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); endometrial cancer (multiple); familial adenomatous polyposis 1; hereditary desmoid disease; and medulloblastoma. Orthologous to human APC (APC regulator of WNT signaling pathway).
• Source: E. coli
• Species: Mouse
• Tag: His&SUMO
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 46.9 kDa
  Accurate Molecular Mass: 58 kDa
• Protein length: Ala2529-Ser2841
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Apc adenomatosis polyposis coli [ Mus musculus (house mouse) ]
• Official Symbol: Apc
• Synonyms: APC; adenomatosis polyposis coli; adenomatous polyposis coli protein; multiple intestinal neoplasia; CC1; Min; mAPC; AI047805; AU020952; AW124434
• Gene ID: 11789
• mRNA Refseq: NM_007462
• Protein Refseq: NP_031488
• UniProt ID: B2RUG9