Recombinant Mouse Angpt1 protein, His-tagged

• Cat.No.: Angpt1-528M
• Product Overview: Recombinant Mouse Angpt1 aa. (Arg35~Gln231) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a secreted glycoprotein that belongs to the angiopoietin family of vascular growth factors. The encoded protein is a ligand in the vascular tyrosine kinase signaling pathway and regulates the formation and stabilization of blood vessels. This protein also functions in striated muscles by promoting proliferation, migration and differentiation of skeletal myoblasts and plays an essential role in the vascular response to tissue injury. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 23kDa as determined by SDS-PAGE reducing conditions
• Protein length: Arg35~Gln231
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 98%
• Characteristic: The isoelectric point is 5.7.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Angpt1 angiopoietin 1 [ Mus musculus (house mouse) ]
• Official Symbol: Angpt1
• Synonyms: Angpt1; angiopoietin 1; Ang1; Ang-1; 1110046O21Rik; angiopoietin-1
• Gene ID: 11600
• mRNA Refseq: NM_001286062.1
• Protein Refseq: NP_001272991.1