Recombinant Mouse AKT3 protein, His&GST-tagged

• Cat.No.: AKT3-0258M
• Product Overview: Recombinant Mouse AKT3 protein(Q9WUA6-1)(Ala106-Glu479), fused with His and GST tag, was expressed in Insect Cells.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 106-479 a.a.
• Form: Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerolPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The recombinant mouse AKT3/GST chimera consists of 611 amino acids and has a calculated molecular mass of 71 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Ala106-Glu479
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Akt3 thymoma viral proto-oncogene 3 [ Mus musculus ]
• Official Symbol: AKT3
• Synonyms: AKT3; thymoma viral proto-oncogene 3; RAC-gamma serine/threonine-protein kinase; PKB gamma; RAC-PK-gamma; protein kinase Akt-3; protein kinase B gamma; Nmf350; AI851531; D930002M15Rik
• Gene ID: 23797
• mRNA Refseq: NM_011785
• Protein Refseq: NP_035915