Recombinant Mouse Akt1 Protein, MYC/DDK-tagged

• Cat.No.: Akt1-566M
• Product Overview: Purified recombinant protein of full-length mouse thymoma viral proto-oncogene 1 (Akt1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes the founding member of the Akt serine-threonine protein kinase gene family that also includes Akt2 and Akt3. This kinase is a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway that mediates the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). It is activated through recruitment to cellular membranes by PI3K lipid products and by phosphorylation by 3-phosphoinositide dependent kinase-1. It then further phosphorylates different downstream proteins in response to various extracellular signals and thus plays a pivotal role in mediating a variety of cellular processes, such as glucose metabolism, glycogen biosynthesis, protein synthesis and turn over, inflammatory response, cell survival (anti-apoptosis) and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Molecular Mass: 56.2 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Akt1 thymoma viral proto-oncogene 1 [ Mus musculus (house mouse) ]
• Official Symbol: Akt1
• Synonyms: Akt1; thymoma viral proto-oncogene 1; Akt; PKB; Rac; LTR-akt; PKB/Akt; PKBalpha; RAC-alpha serine/threonine-protein kinase; AKT1 kinase; Akt1m protein; PKB alpha; RAC-PK-alpha; protein kinase B-alpha; proto-oncogene c-AKT; related to A and C kinases; tumor rejection antigen peptide pRLib; EC 2.7.11.1
• Gene ID: 11651
• mRNA Refseq: NM_009652
• Protein Refseq: NP_033782
• UniProt ID: P31750