Recombinant MERS N protein, His-tagged

• Cat.No.: N-0562M
• Product Overview: Recombinant MERS N protein(Met1-Asp413), fused with His tag, was expressed in HEK293.

Specification

• Species: MERS-CoV
• Source: HEK293
• Tag: His
• Protein Length: 1-413 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin cleavage site, 251-RHKR-254, and is partially processed into N and C-terminal fragment with calculated MW of 17.1 kDa and 19.7 kDa respectively. The protein migrates as 23-25 kDa (C-terminus) and 46-73 kDa (full length) under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Met1-Asp413
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.