Recombinant MERS-CoV Spike S1+S2 protein(Met1-Trp1297), His-tagged, Biotinylated

Cat.No. : Spike-1227V
Product Overview : Recombinant MERS-CoV spike protein (AFS88936.1) (Met1-Trp1297) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
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Species : MERS-CoV
Source : Insect Cells
Tag : His
Protein Length : Met1-Trp1297
Conjugation/Label : Biotin
Form : Lyophilized from sterile PBS (contains 300 mM NaCl), pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant extracellular domain of spike protein MERS-CoV comprises 1291 amino acids and has a predicted molecular mass of 142.52 kDa. It migrates as an approximately 157.6 kDa band in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 80 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Not For Human Consumption!

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