Recombinant MERS-CoV Spike/RBD protein(Glu367-Tyr606), His-tagged

• Cat.No.: Spike-1229V
• Product Overview: Recombinant MERS-CoV receptor binding domain (RBD) of spike protein fragment (AFS88936.1) (Glu367-Tyr606) was expressed in Insect Cells with a polyhistidine tag at the C-terminus.

Specification

• Source: Insect Cells
• Species: MERS-CoV
• Tag: His
• Protein length: Glu367-Tyr606
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant receptor binding domain (RBD) of spike protein fragment MERS-CoV consists of 251 amino acids and predicts a molecular mass of 27.7 kDa. As a result of glycosylation, it migrates as an approximately 33.7 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.