Recombinant MERS-CoV S Protein (Glu366-Asn602), C-His tagged

• Cat.No.: S-5395M
• Product Overview: Recombinant MERS-CoV S/Spike glycoprotein (Glu366-Asn 602) fused with the C-terminal His tag was expressed in Mammalian cells.

Specification

• Species: MERS-CoV
• Source: Mammalian Cells
• Tag: His
• Protein Length: Glu366-Asn602
• Description: Spike protein S1: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.
  Spike protein S2: Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.
  Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 30.72 kDa
• Purity: >90% as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS pH 7.4.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: S spike protein [ Middle East respiratory syndrome-related coronavirus ]
• Official Symbol: S
• Synonyms: S; spike protein; surface glycoprotein
• Gene ID: 14254594
• Protein Refseq: YP_009047204
• UniProt ID: A0A140AYZ5