Recombinant MERS-CoV N Protein (Met1-Thr411), C-6×His-tagged

• Cat.No.: N-01M
• Product Overview: Recombinant MERS-CoV N Protein (Met1-Thr411) with a C-terminal 6-His tag was expressed in Spodoptera frugiperda, Sf 21 (baculovirus).

Specification

• Species: MERS-CoV
• Source: Insect Cells
• Tag: His
• ProteinLength: Met1-Thr411
• Description: MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N). While the S, E and M proteins build up the viral envelope, the N protein is involved transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP). The MERS-CoV N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. The N-terminal region contains an Intrinsically Disordered Region and an RNA binding domain, the linker region interacts with the M protein and the C-terminal region contains a self-association domain. The MERS-CoV N protein shares 46.3% and 4.5% amino acid sequence identity with SARS-CoV-1 and SARS-CoV-2 N protein, respectively. MERS-CoV N proteins have been shown to inhibit Type I Interferon(IFN) production. In addition, the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity, making it a promising therapeutic target.
• Bio-activity: Bioassay data are not available.
• Molecular Mass: Predicted Molecular Mass: 46 kDa
  SDS-PAGE: 49-55 kDa, under reducing conditions
• N-terminal Sequence Analysis: Sequence has been confirmed by mass spectrometry
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: N nucleocapsid protein [ Middle East respiratory syndrome-related coronavirus ]
• Official Symbol: N
• Synonyms: N; nucleocapsid protein; nucleocapsid protein; nucleoprotein
• Gene ID: 14254601
• Protein Refseq: YP_009047211
• UniProt ID: K0BVN3