Recombinant MERS-CoV N protein(Met1-Asp413), His-tagged

• Cat.No.: N-3308V
• Product Overview: Recombinant MERS-CoV Nucleoprotein (AFS88943.1) (Met1-Asp413) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: MERS-CoV
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Asp413
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Nucleoprotein MERS-CoV comprises 424 amino acids and has a predicted molecular mass of 46.51 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.