Recombinant Influenza B (B/PHUKET/3073/2013) HA protein(Met1-Thr547), His-tagged

• Cat.No.: HA-2433I
• Product Overview: Recombinant Influenza virus B (B/PHUKET/3073/2013) hemagglutinin (translated amino acids of EPI529345) (Met1-Thr547), termed as HA, was expressed in Insect Cells with a polyhistidine tag at the C-terminus.

Specification

• Species: Influenza B
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Thr547
• Form: Lyophilized from sterile 20 mM PB, pH 7.0, 500 mM NaCl, 10 % glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.5-5 μg/mL for 1%GRBC.
• Molecular Mass: The recombinant hemagglutinin of Influenza virus B (B/PHUKET/3073/2013) consists of 543 amino acids and predicts a molecular mass of 59.1 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.