Recombinant Influenza B (B/Florida/4/2006) HA protein(Gly362-Ala555), mFc-tagged

• Cat.No.: HA-2419I
• Product Overview: Recombinant influenza B virus (B/Florida/4/2006) (ACA33493.1)(Gly 362-Ala 555) hemagglutinin, termed as HA2, was expressed in HEK293, fused with the Fc region of Mouse IgG1 at the N-terminus.

Specification

• Species: Influenza B
• Source: HEK293
• Tag: mFc
• Protein Length: Gly362-Ala555
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Influenza B virus (B/Florida/4/2006) HA2/mFc is a disulfide-linked homodimeric protein. The reduced monomer comprises 430 amino acids with the predicted molecular mass of 47.6 kDa. As a result of glycosylation, it migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.