Recombinant Influenza A H9N2 (A/Guinea fowl/Hong Kong/WF10/99) HA1 Protein, His & Avi-tagged, Biotinylated

• Cat.No.: HA1-348I
• Product Overview: Recombinant Influenza A H9N2 (A/Guinea fowl/Hong Kong/WF10/99) HA1 Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Arg 338 (Accession # Q80KD9-1). Predicted N-terminus: Asp 19. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 39.3 kDa. The protein migrates as 47-70 kDa under reducing (R) condition (SDS-PAGE). The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Species: Influenza A H9N2
• Source: HEK293
• Tag: Avi&His
• Protein Length: Asp 19 - Arg 338
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Molecular Mass: 39.3 kDa
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.