Recombinant Influenza A H7N9 (A/Anhui/1/2013) HA protein(Met1-Gly338), His-tagged

• Cat.No.: HA-1111V
• Product Overview: Recombinant Influenza A virus (A/Anhui/1/2013(H7N9)) hemagglutinin (AGJ51953.1) (Met1-Gly338), termed as HA1, was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: H7N9
• Source: HEK293
• Tag: His
• Protein Length: Met1-Gly338
• Form: Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant HA1 subunit of Influenza A virus (A/Anhui/1/2013 (H7N9)) hemagglutinin comprises 331 amino acids and has a predicted molecular mass of 36.2 kDa. The apparent molecular mass of the protein is approximately 39-47 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.