Recombinant Influenza A H6N1 (A/northern shoveler/California/HKWF115/2007) HA protein(Met1-Arg344), His-tagged

• Cat.No.: HA-3277V
• Product Overview: Recombinant Influenza A hemagglutinin (A/northern shoveler/California/HKWF115/2007 (H6N1)) (ACE81692.1)(Met 1-Arg 344), termed as HA1 was expressed in HEK293, fused with a C-terminal polyhistidine tag.

Specification

• Species: H6N1
• Source: HEK293
• Tag: His
• Protein Length: Met1-Arg344
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant influenza A H6N1 HA1 subunit (A/northern shoveler/California/HKWF115/2007 (H6N1)) comprises 339 amino acids and has a predicted molecular mass of 38 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 45-55 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.