Recombinant Influenza A H5N1 (A/Thailand/1(KAN-1)/2004) NA protein(His36-Ile469), His-tagged, Biotinylated

• Cat.No.: NA-1072V
• Product Overview: Biotinylated Recombinant Influenza A virus (A/Thailand/1(KAN-1)/2004(H5N1)) neuraminidase (His36-Ile469), termed as NA, was expressed in HEK293, fused with a N-terminal polyhistidine tag.

Specification

• Species: H5N1
• Source: HEK293
• Tag: His
• Protein Length: His36-Ile469
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant NA subunit of influenza A H5N1 (A/Thailand/1(KAN-1)/2004(H5N1)) comprises 433 amino acids and has a predicted molecular mass of 47.6 kDa. It migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 65 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.