Recombinant Influenza A H5N1 (A/Indonesia/5/2005) HA protein, His-tagged

• Cat.No.: HA-740I
• Product Overview: Recombinant Influenza A H5N1 (A/Indonesia/5/2005) HA protein(ABW06108.1)(Met 1-Gln 531) (Native, HA1+HA2, cleaved) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: H5N1
• Source: HEK293
• Tag: His
• Protein Length: Met 1-Gln 531
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to agglutinate guinea pig red blood cells.
  HA titer is 0.3-1.5 μg/mL for 1% GRBC.
  It also agglutinates chick red blood cells.
• Molecular Mass: The secreted recombinant hemagglutinin of Influenza A virus H5N1 (A/Indonesia/5/2005) comprises 526 amino acids with the predicted molecular mass of 60 kDa. As a result of glycosylation, it migrates as an approximately 65-70 kDa band in Non-reduced SDS-PAGE, and three bands (25, 45, 70 kDa) in reduced SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.