Recombinant Influenza A H1N1 (A/Wisconsin/588/2019) / (A/Victoria/2570/2019) NP protein(Met1-Asn498), His-tagged

• Cat.No.: NP-3358V
• Product Overview: Recombinant Influenza A virus ((A/Wisconsin/588/2019) / (A/Victoria/2570/2019) (H1N1)) nucleoprotein (translated amino acids of EPI1661751 / EPI1799574) (Met1-Asn498) was expressed in Insect Cells with a polyhistidine tag at the C-terminus.

Specification

• Species: H1N1
• Source: Insect Cells
• Tag: His
• ProteinLength: Met1-Asn498
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant nucleoprotein of Influenza A virus ((A/Wisconsin/588/2019) / (A/Victoria/2570/2019) (H1N1)) consists of 509 amino acids and predicts a molecular mass of 57.5 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.