Recombinant Influenza A H1N1 (A/California/04/2009) HA protein(Met1-Gln529), His-tagged

• Cat.No.: HA-283V
• Product Overview: Recombinant Influenza A H1N1 (A/California/04/2009) HA protein(ACP41105.1)(Met1-Gln529), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: H1N1
• Source: HEK293
• Tag: His
• Protein Length: Met1-Gln529
• similar: HA
• Alias: HA
• Conjugate: C-His
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• MolecularMass: The secreted recombinant Influenza A virus (A/California/04/2009 (H1N1)) HA (mutated) comprises 527 amino acids and predicts a molecular mass of 60 kDa. As a result of glycosylation and proteolysis, the apparent molecular mass of the hemagglutinin is apparoximately 25-30, 50-55 and 80-85 kDa in SDS-PAGE under reducing conditions, corresponding to the HA2, HA1 subunit and the full length HA respectively.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• OfficialSymbol: HA
• Publications:
  Variation of Site-Specific Glycosylation Profiles of Recombinant Influenza Glycoproteins (2024)

Gene Information