Recombinant Influenza A H1N1 (A/Brisbane/59/2007) HA protein(Met1-Arg343), His-tagged

• Cat.No.: HA-3276V
• Product Overview: Recombinant Influenza A virus (A/Brisbane/59/2007 (H1N1)) (ACA28844.1) HA(Met 1-Arg 343), termed as HA1 subunit, was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: H1N1
• Tag: His
• Protein length: Met1-Arg343
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: Recombinant HA1 subunit of Influenza A virus (A/Brisbane/59/2007 (H1N1)) hemagglutinin consists of 337 amino acids and has a calculated molecular mass of 37.7 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.