Recombinant Influenza A [A/Hong Kong/483/97 (H5N1)] HA protein, His-tagged

• Cat.No.: HA-344V
• Product Overview: Recombinant Influenza A [A/Hong Kong/483/97 (H5N1)] HA (Asp17-Gln531) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.
• Source: HEK293
• Species: Influenza
• Tag: His
• Predicted N Terminal: Asp17
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 60.3 kDa. The protein migrates as 66-100 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: Asp17-Gln531
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: HA
• Official Symbol: HA
• Synonyms: FLUAVH5N1_s4gp1
• Gene ID: 3654620
• Protein Refseq: YP_308669.1
• UniProt ID: O92930