Recombinant IBV NP, His-tagged

• Cat.No.: INF-461
• Product Overview: Influenza B [B/Beijing/184/93] nucleoprotein (NP) is a recombinant antigen expressed and purified to high purity from Escherichia coli.

Specification

• Tag: His
• Background: Of the three types of influenza virus (A, B and C) A is the best characterized and the most serious threat to public health. One of the three core Influenza polypeptides is an RNA-dependent RNA polymerase and a single-strand RNA (ssRNA) binding protein, often referred to as a nucleoprotein (NP). NP encapsidates the virus genome to form a ribonucleoprotein (RNP) particle for the purposes of transcription and packaging. NP is not just the structural component of the virus transcription machinery but performs multiple essential functions throughout the virus life cycle (Portela & Digard, 2002). Influenza A virus RNA segment 5 encodes NP is a polypeptide of 498 amino acids in length; in influenza B and C viruses, the length of the homologous NP polypeptide is 560 and 565 residues, respectively (Londo et al., 1983; Nakada et al., 1984). Alignment of the predicted amino acid sequences of the NP genes of the three influenza virus types reveals significant similarity among the three proteins, with the type A and B NPs showing the highest degree of conservation. Phylogenetic analysis of virus strains isolated from different hosts reveals that the NP gene is also well conserved, with a maximum amino acid difference of less than 11% (Shu et al., 1993). The primary sequence of NP is modified by phosphorylation (Privalsky & Penhoet, 1978) and the phosphopeptide pattern of NP is strain-specific, dependent on the host cell used for propagating the virus and changes throughout virus replication (Kistner et al., 1985, 1989). Seasonal influenza viruses display high variability and because of the threat of influenza viruses with pandemic potential, there is great interest in the development of vaccines that induce broadly protective immunity, such as those based on conserved proteins, including nucleoprotein. NP has also been shown to induce cross-reactive antibody and T cell responses (Altenburg et al., 2017).
• Purity: >90% purity
• Formulation: 50mM Tris-HCl, 400mM NaCl, 2M Urea pH8.0
• Notes: This product is intended for research and manufacturing uses only. It is not a diagnostic device. The user assumes all responsibility for care, custody and control of the material, including its disposal, in accordance with all regulations.
• Type: Recombinant
• ClassID 1: Infectious Disease

Gene Information

• Synonyms: Influenza B [B/Beijing/184/93] Nucleoprotein (NP)