Recombinant Human XPO1 Protein (Thr917-Asp1071), His tagged

• Cat.No.: XPO1-5164H
• Product Overview: Recombinant human XPO1 (Thr917-Asp1071) fused with His tag was expressed in E. coli.

Specification

• Description: Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP) . Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 18.66 kDa
• Protein length: Thr917-Asp1071
• Purity: >90% as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS, pH 7.5 or lyophilized from PBS, pH 7.5.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: XPO1 exportin 1 (CRM1 homolog, yeast) [ Homo sapiens (human) ]
• Official Symbol: XPO1
• Synonyms: XPO1; exportin 1 (CRM1 homolog, yeast); exportin 1 (CRM1, yeast, homolog); exportin-1; CRM1; emb; CRM1, yeast, homolog; chromosome region maintenance 1 protein homolog; exportin-1 (required for chromosome region maintenance); exp1; DKFZp686B1823
• Gene ID: 7514
• mRNA Refseq: NM_003400
• Protein Refseq: NP_003391
• MIM: 602559
• UniProt ID: O14980