Recombinant Human WIF1 protein, His-tagged

• Cat.No.: WIF1-30862TH
• Product Overview: Recombinant Human WIF1(29-379aa) fused with His tag at C-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 29-379 a.a.
• Description: Wnt inhibitory factor 1, also known as Wif-1, is a secreted protein that binds to Wnt proteins involved in the embryonic development and cancer, and inhibits their activity. Wif-1 protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 20% glycerol, 0.1mM PMSF, 1mM DTT
• Molecular Mass: 39.5 kDa (360 aa) 40-57 KDa (SDS-PAGE under reducing conditions)
• AA Sequence: ADLGPPQEES LYLWIDAHQA RVLIGFEEDI LIVSEGKMAP FTHDFRKAQQ RMPAIPVNIH SMNFTWQAAG QAEYFYEFLS LRSLDKGIMA DPTVNVPLLGTVPHKASVVQ VGFPCLGKQD GVAAFEVDVI VMNSEGNTIL QTPQNAIFFK TCQQAECPGG CRNGGFCNER RICECPDGFH GPHCEKALCT PRCMNGGLCVTPGFCICPPG FYGVNCDKAN CSTTCFNGGT CFYPGKCICP PGLEGEQCEI SKCPQPCRNG GKCIGKSKCK CSKGYQGDLC SKPVCEPGCG AHGTCHEPNKCQCQEGWHGR HCNKRYEASL IHALRPAGAQ LRQHTPSLKK AEERRDPPES NYIWHHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.20mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: WIF1 WNT inhibitory factor 1 [ Homo sapiens ]
• Official Symbol: WIF1
• Synonyms: WIF1; WNT inhibitory factor 1; wnt inhibitory factor 1; WIF-1
• Gene ID: 11197
• mRNA Refseq: NM_007191
• Protein Refseq: NP_009122
• MIM: 605186
• UniProt ID: Q9Y5W5