Recombinant Human VRK1 Protein (Full Length), N-His-tagged

• Cat.No.: VRK1-31740TH
• Product Overview: Recombinant protein of human vaccinia related kinase 1 (VRK1), full length, with N-terminal HIS tag, expressed in E. coli.

Specification

• Description: This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 45.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl.
• Full Length: Full L.

Gene Information

• Gene Name: VRK1 VRK serine/threonine kinase 1 [ Homo sapiens (human) ]
• Official Symbol: VRK1
• Synonyms: VRK1; VRK serine/threonine kinase 1; PCH1; PCH1A; serine/threonine-protein kinase VRK1; vaccinia related kinase 1; vaccinia virus B1R-related kinase 1; EC 2.7.11.1
• Gene ID: 7443
• mRNA Refseq: NM_003384
• Protein Refseq: NP_003375
• MIM: 602168
• UniProt ID: Q99986