Recombinant Human Vesicle-associated Membrane Protein 4, His-tagged

• Cat.No.: VAMP4-484H
• Product Overview: Recombinant human VAMP4 protein, fused to His-tag at C-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 14.5 kDa (123aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: VAMP4, also known as vesicle-associated membrane protein 4, is a member of the synaptobrevin family involved in docking and/or fusion of vesicles with cell membrane. This protein is enriched in the trans-Golgi network and may play a role in trans-Golgi network-to-endosome transport. VAMP4 is involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules.
• Amino Acid Sequence: MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN RSKQLRRQMW WRGCKLEHHH HHH.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, and 20% glycerol.
• Purity: > 90% by SDS – PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method ).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: VAMP4 vesicle-associated membrane protein 4 [ Homo sapiens ]
• Synonyms: VAMP4; vesicle-associated membrane protein 4; VAMP24;VAMP-4
• Gene ID: 8674
• mRNA Refseq: NM_003762
• Protein Refseq: NP_003753
• MIM: 606909
• UniProt ID: O75379
• Chromosome Location: 1q24-q25
• Pathway: SNARE interactions in vesicular transport