Recombinant Human Vesicle Amine Transport Protein 1 Homolog (T. californica), His-tagged

• Cat.No.: VAT1-1339H
• Product Overview: Recombinant human VAT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: VAT1 (Synaptic vesicle membrane protein VAT-1 homolog) belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins. Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. This protein increased expression in glioblastomas and on wounding, in basal keratinocytes. This expression is calcium ion-dependent.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol, 100 mM NaCl.
• Molecular Weight: 44.1 kDa (413 aa), confirmed by MALDI-TOF
• Purity: > 90 % by SDS-PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSDEREVAEA ATGEDASSPP PKTEAASDPQ HPAASEGAAA AAASPPLLRC LVLTGFGGYD KVKLQSRPAA PPAPGPGQLT LRLRACGLNF ADLMARQGLY DRLPPLPVTP GMEGAGVVIA VGEGVSDRKA GDRVMVLNRS GMWQEEVTVP SVQTFLIPEA MTFEEAAALL VNYITAYMVL FDFGNLQPGH SVLVHMAAGG VGMAAVQLCR TVENVTVFGT ASASKHEALK ENGVTHPIDY HTTDYVDEIK KISPKGVDIV MDPLGGSDTA KGYNLLKPMG KVVTYGMANL LTGPKRNLMA LARTWWNQFS VTALQLLQAN RAVCGFHLGY LDGEVELVSG VVARLLALYN QGHIKPHIDS VWPFEKVADA MKQMQEKKNV GKVLLVPGPE KEN
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: VAT1 vesicle amine transport protein 1 homolog (T. californica) [ Homo sapiens ]
• Official Symbol: VAT1
• Synonyms: VAT1; vesicle amine transport protein 1 homolog (T. californica); VATI; FLJ20230; synaptic vesicle membrane protein VAT-1 homolog; membrane protein of cholinergic synaptic vesicles
• Gene ID: 10493
• mRNA Refseq: NM_006373
• Protein Refseq: NP_006364
• MIM: 604631
• UniProt ID: Q99536
• Chromosome Location: 17q21
• Function: binding; oxidoreductase activity; zinc ion binding