Recombinant Human VEGFA, His-tagged

• Cat.No.: VEGFA-31560TH
• Product Overview: Recombinant full length mature Human VEGF 165A with His tag at N-terminalproduced in the endosperm tissue of barley grain ; 181 amino acids with tag, Predicted MWt 20.9 kDa.

Specification

• Species: Human
• Tag: His
• Description: This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.
• Conjugation: HIS
• Tissue specificity: Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
• Biological activity: Bioactivity: The ED50 is determined by Cell Proliferation Assay for the dose-dependent effects of serial dilutions of recombinant Human VEGF 165A on Human Umbilical Vein Endothelial Cells (HUVEC) cells. The ED50 value for VEGFA-31560TH was determined to b
• Form: Lyophilised:Reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 μg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA).
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: PBS, pH 7.2
• Storage: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.
• Sequence Similarities: Belongs to the PDGF/VEGF growth factor family.
• Full Length: Full L.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF
• Gene ID: 7422
• mRNA Refseq: NM_001025366
• Protein Refseq: NP_001020537
• Uniprot ID: P15692
• Chromosome Location: 6p12
• Pathway: Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Endochondral Ossification, organism-specific biosystem
• Function: cell surface binding; chemoattractant activity; cytokine activity; cytokine activity; extracellular matrix binding