Recombinant Human Vascular Endothelial Growth Factor A

• Cat.No.: VEGFA-709H
• Product Overview: Recombinant human VEGFA,a 18 kDa protein consisting of 121 amino acid residues is produced as ahomodimer. VEGFA is a polypeptide growth.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Description: VEGFA is apolypeptide growth factor and a member of the platelet-derived growth factorfamily. It is a specific mitogen for vascular endothelial cells and a strongangiogenic factor in vivo. Two high-affinity tyrosine kinase receptors forVEGF121 have been identified, VEGFA (FLT-1), and VEGFR-2 (KDR).Consistent with the endothelial cell-specific action of VEGF121, expressionof both receptor genes has been found predominantly but not exclusively on endothelialcells. Expression of VEGFA was also found on human monocytes,neutrophils(PMNs), bovine brain pericytes and villous and extravillous trophoblasts.
• Molecule weight: 18 kDa
• Form: Lyophilized
• Purity: > 90%, bySDS-PAGE and visualized by silver stain
• Endotoxin Level: < 0.1 ng per µgof VEGF
• Biological Activity: The ED50 forstimulation of 3 H-thymidine incorporation and cell proliferation by human umbilicalvein endothelial cells for VEGF121 has been determined to be in the range of 2-5ng/ml.
• Specific Activity: 5 x 105units/mg
• Reconstitution: Centrifuge vialprior to opening. The lyophilized VEGF121 is soluble in water andmost aqueous buffers. The lyophilized VEGF121 should bereconstituted in PBS or medium containing at least 0.1% human or bovine serumalbumin to a concentration not lower than 50 µg/ml.
• Storage: Lyophilized samplesare stable for greater than six months at -20°C to -70°C. Reconstituted VEGF121should be stored in working aliquots at -20°C. Avoid repeated freeze-thawcycles.
• OfficialSymbol: VEGFA

Gene Information

• Gene Name: VEGFA vascular endothelialgrowth factor A [ Homo sapiens ]
• Synonyms: VEGFA; vascularendothelial growth factor A; VPF; VEGF; MVCD1; OTTHUMP00000016487;OTTHUMP00000016488; OTTHUMP00000165985; OTTHUMP00000165986;OTTHUMP00000165987; OTTHUMP00000224107; OTTHUMP00000224108;OTTHUMP00000224109; OTTHUMP00000224153; OTTHUMP00000224154;OTTHUMP00000224423; OTTHUMP00000224424; OTTHUMP00000224425;OTTHUMP00000224426; OTTHUMP00000224427; OTTHUMP00000224428;OTTHUMP00000224429; OTTHUMP00000224430; vascular permeability factor
• Gene ID: 7422
• mRNA Refseq: NM_003376
• Protein Refseq: NP_003367
• MIM: 192240
• UniProt ID: P15692
• Chromosome Location: 6p12
• Pathway: Bladder cancer;Cytokine-cytokine receptor interaction; Endochondral Ossification; Focaladhesion; Glypican 1 network; HIF-1-alpha transcription factor network; IdSignaling Pathway; Neurophilin interactions with VEGF and VEGFR; Pancreaticcancer; Renal cell carcinoma; Signal Transduction; Signaling events mediatedby VEGFR1 and VEGFR2; VEGF ligand-receptor interactions; VEGFR1 specificsignals; mTOR signaling pathway
• Function: cell surfacebinding; chemoattractant activity; cytokine activity; extracellular matrixbinding; fibronectin binding; growth factor activity; heparin binding;platelet-derived growth factor receptor binding; protein binding; proteinheterodimerization activity; vascular endothelial growth factor receptor 1binding; vascular endothelial growth factor receptor 2 binding; vascularendothelial growth factor receptor binding