Recombinant Human VARS protein, His & T7-tagged

• Cat.No.: VARS-8221H
• Product Overview: Recombinant Human VARS aa. (Ser2~Gln219 (Accession # P26640)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Ser2~Gln219
• Description: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.9kDa.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 8.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: VARS valyl-tRNA synthetase [ Homo sapiens (human) ]
• Official Symbol: VARS
• Synonyms: VARS; valyl-tRNA synthetase; G7A; VARS1; VARS2; valine--tRNA ligase; protein G7a; valRS; valine tRNA ligase 1, cytoplasmic; valyl-tRNA synthetase 2
• Gene ID: 7407
• mRNA Refseq: NM_006295.2
• Protein Refseq: NP_006286.1
• UniProt ID: P26640