Recombinant Human UDP glycosyltransferase 8, His-tagged

• Cat.No.: UGT8-331H
• Product Overview: UGT8, 21-541aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 21-541 a.a.
• Description: UDP glycosyltransferase 8, also known as UGT8, catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system. Recombinant human UGT8 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea
• Molecular Mass: 61.6 kDa (544aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSAKIIIVP PIMFESHMYI FKTLASALHE RGHHTVFLLS EGRDIAPSNH YSLQRYPGIF NSTTSDAFLQ SKMRNIFSGR LTAIELFDIL DHYTKNCDLM VGNHALIQGL KKEKFDLLLV DPNDMCGFVI AHLLGVKYAV FSTGLWYPAE VGAPAPLAYV PEFNSLLTDR MNLLQRMKNT GVYLISRLGV SFLVLPKYER IMQKYNLLPE KSMYDLVHGS SLWMLCTDVA LEFPRPTLPN VVYVGGILTK PASPLPEDLQ RWVNGANEHG FVLVSFGAGV KYLSEDIANK LAGALGRLPQ KVIWRFSGPK PKNLGNNTKL IEWLPQNDLL GHSKIKAFLS HGGLNSIFET MYHGVPVVGI PLFGDHYDTM TRVQAKGMGI LLEWKTVTEK ELYEALVKVI NNPSYRQRAQ KLSEIHKDQP GHPVNRTIYW IDYIIRHNGA HHLRAAVHQI SFCQYFLLDI AFVLLLGAAL LYFLLSWVTK FIYRKIKSLW SRNKHSTVNG HYHNGILNGK YKRNGHIKHE KKVK
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: UGT8?UDP glycosyltransferase 8 [?Homo sapiens?(human) ]
• Official Symbol: UGT8
• Synonyms: UGT8; CGT; UGT4; UDP glycosyltransferase 8; 2-hydroxyacylsphingosine; 1-beta-galactosyltransferase; cerebroside synthase; ceramide UDP-galactosyltransferase; uridine diphosphate glycosyltransferase 8; UDP-galactose ceramide galactosyltransferase; UDP-galactose-ceramide galactosyltransferase; NP_001121646.1; EC 2.4.1.45; NP_003351.2
• Gene ID: 7368
• mRNA Refseq: NM_001128174
• Protein Refseq: NP_001121646
• MIM: 601291
• UniProt ID: Q16880
• Chromosome Location: 4q26
• Pathway: Cerebroside and sulfatide biosynthesis; Sphingolipid metabolism
• Function: 2-hydroxyacylsphingosine 1-beta-galactosyltransferase activity; UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity